17年間の米国での研究生活では、ニューヨーク州立大学、フロリダ大学、NIHなどに在籍し、共同研究をつうじて医学生物学のさまざまな分野にかかわってきました。フロリダ大学、NIHではPIとして自分のラボを運営しましたが、そこでのプロジェクトとしては、小型の脊椎動物であるZebrafishを実験系として、主に神経筋接合部をモデルとしたシナプスの研究を行ってきました。具体的には、アセチルコリン受容体の変異体を解析する事により、シナプス形成において受容体が能動的な役割を果たすこと、筋細胞の種類（速筋、遅筋）に応じて受容体の性質や組成が異なることなどを明らかにしました。2014年に赴任した大阪医大では、意欲ある優秀なスタッフに恵まれ、今までの研究をさらに発展させると同時に、新規の分野にもチャレンジしてきました。2020年には、遅筋だけが運動神経の入力を受ける動物を世界で初めて作成して解析した仕事や、一般消化器外科との共同研究で消化管逆蠕動のメカニズムを初めて明らかにした仕事なども論文になり、大阪医大での研究も形になり始めてきていると感じています。また研究と同時に学部の生理学の授業でも、学生に生理学の楽しさを伝えていきたいと思っています。臨床にすすむ学生であっても、生理学を理解することは将来の学習、研究、診療に必ず役にたつものと思います。
小野富三人
Fumihito Ono, M.D., Ph.D.
学歴、職歴
1991 M.D., Faculty of Medicine, University
of Tokyo
1991 Resident, Internal Medicine, National
Medical Center Hospital, Tokyo
1996 Ph.D., Faculty of Medicine,
University of Tokyo
1996 Postdoctoral Fellow of the Japan
Society for the Promotion of Science
1998 Postdoctoral Fellow, SUNY at Stony
Brook
2000 Research Assistant Professor, SUNY at
Stony Brook
2003 Assistant Professor, the Whitney
Laboratory for Marine Bioscience & Department of Neuroscience, University
of Florida
2007 Chief, Section on Model Synaptic
Systems, Laboratory of Molecular Physiology, NIAAA/NIH
2014-Present Professor/Chair, Department of
Physiology, Osaka Medical College
2015-Present Director, Research Center, Osaka Medical
College
2018-Present Vice President, Osaka Medical College
免許、資格
1991 ECFMG certification (No. 444-893-2)
The Basic Medical Science Component Passed
The Clinical Science Component Passed
The ECFMG English test Passed
1991 医師免許 (No. 341898)
賞罰
1996-1998 Postdoctoral Fellowship,
Japan Society for the Promotion of Science
2004-2007 Research Grant, Muscular
Dystrophy Association
2005-2009 R01 grant (NS050388-01A1),
NIH
2014 NIH Special Act Award
2016 Best Teacher
Award, Osaka Medical College
2018 Best
Teacher Award, Osaka Medical College
2018-2021 Grant-In-Aid for
Scientific Research (KAKENHI 18K06882)
教育
大阪医科大学医学部 ２年生 生理学講義、生理学実習
大阪医科大学看護学部 １年生 からだの仕組みと働き講義
高槻市医師会看護専門学校 解剖生理学II講義
履正社専門学校 生理学講義
大阪医科大学華道部顧問 2015-現在
大阪医科大学国際交流部部長 2018-現在
大阪医科大学医学クイズ研究会顧問 2018-現在
USMLE STEP1勉強会 2018-現在
書籍
『ガイトン生理学』（エルセビアジャパン） 第１３版 第１２章 共訳
『カラー図解 よくわかる生理学の基礎』 （メディカルサイエンスインターナショナル） 第１２章 共訳
『僕たちが医者になるまえに』 （カイ出版）荘子万能、徳田安春著 第４章、第５章 インタビュー記事
論文、総説
Zempo B, Yamamoto, Y., Williams, T., Ono, F. (2020) Synaptic silencing of fast muscle is compensated by rewired innervation of slow muscle. Science Advances In Press
Fujii, K.,
Nakajo, K., Egashira, Y., Yamamoto, Y., Kitada,
K., Taniguchi, K., Kawai, M., Tomiyama, H., Kawakami,
K., Uchiyama, K., Ono, F. (2020) Gastrointestinal
Neurons Expressing HCN4 Regulate Retrograde Peristalsis. Cell Reports 30:2879-2888
Li L et al. (3rd in 41 authors) (2018) Mutation in the intracellular chloride channel CLCC1 associated with autosomal recessive retinitiss pigmentosa. PLoS Genet 14:e1007504.
Egashira, Y., Zempo, B., Sakata, S., Ono, F. (2018) Recent advances in
neuromuscular junction research prompted by the zebrafish model. Current Opinion in Physiology 04:70-75.
Shiraiwa,
Y., Daikoku. E., Saito, M., Yamashita, Y., Abe, T., Ono, F., Kubota, T.
(2018) Measurements of ionic concentrations along with endocochlear
potential in wild-type and claudin 14 KO mice. Auris Nasus
Larynx 45:421-426.
Taruno. A., Sun, H., Nakajo, K., Murakami, T., Ohsaki, Y., Kido, M., Ono, F., Marunaka, Y. (2017) Post-translational palmitoylation
controls the voltage gating and lipid raft association of the CALHM1 channel. Journal of Physiology 595:
6121-6145.
Mott, M., Luna, V., Park, J., Downes, G., Epley, K., Ono, F. (2017) Expressing acetylcholine receptors after
innervation suppresses spontaneous vesicle release and causes muscle fatigue. Scientific Reports 7:
1674.
Nishino, A., Ono, F. (2017) Neuromuscular synapses in ascidians, fish and higher vertebrates. Comparative Physiology and Biochemistry 34:12-21. (Japanese)
Torvund, M., Ma, T., Connaughton, V., Ono, F., Nelson, R. (2016) Cone signals
in the monostratified and bistratified amacrine cells of adult zebrafish retina. Journal of Comparative Neurology doi:10.1002/cne.24107.
Luna, V., Daikoku, E., Ono, F. (2015) “Slow” muscles across
vertebrate species. Cell & Bioscience 5:62.
Ono, F., Daikoku, E., Saito, M. (2015) Zebrafish as a model synaptic system. The Journal of Osaka Medical College 74:1-6. (Japanese)
Daikoku, E., Saito, M., Ono,
F. (2015) Zebrafish mutants of the neuromuscular junction: Swimming in the gene
pool. Journal of Physiological Sciences 65: 217-221.
Park, J., Mott, M., Williams, T., Ikeda, H., Wen, H., Linhoff, M., Ono, F. (2014) A single mutation in the acetylcholine receptor delta-subunit causes distinct effects in two types of neuromuscular synapses. Journal of Neuroscience 34: 10211-10218.
Puhl, H., Lu, V.,
Won, Y., Sasson, Y., Hirsch, J., Ono, F.,
Ikeda, S. (2014) Ancient origins of RGK protein function: modulation of
voltage-gated calcium channels preceded the protostome and
deuterostome split. PLoS ONE 9: e100694.
Fero, K., Bergeron, S., Horstick, E., Codore, H., Li, G., Ono, F., Dowling, J., Burgess, H. (2014) Impaired embryonic motility in dusp27 mutants reveals a developmental defect in myofibril structure. Disease Models and Mechanisms 7: 289-298.
Ikeda, H., Delargy, A., Yokogawa, T., Urban, J., Burgess, H., Ono, F. (2013) Intrinsic properties of larval zebrafish neurons in ethanol. PLoS ONE 8: e63318.
Ono, F. (2013) Big answers from a small fly with a cultured brain. Journal of Physiology 591: 3.
Walogorsky, M., Wen, H., Mongeon, R., Urban, J., Ono, F., Mandel, G., Brehm, P. (2012) A zebrafish model for congenital myasthenic syndrome reveals mechanisms causal to developmental recovery. Proceedings of the National Academy of Sciences 109: 17711-17716.
Won, Y., Ono, F., Ikeda, S. (2012) Characterization of Na+ and Ca2+ channels in zebrafish dorsal root ganglion neurons. PLoS ONE 7: e42602.
Papke, R., Ono, F., Stokes, C., Urban, J., Boyd, T. (2012) The nicotinic acetylcholine receptors of zebrafish and an evaluation of pharmacological tools used for their study. Biochemical Pharmacology 84:352-365.
Park, J., Ikeda, H, Ikenaga, T., Ono, F. (2012) Acetylcholine receptors enable the transport of rapsyn from the Golgi complex to the plasma membrane. Journal of Neuroscience 32:7356-7363.
Nakayama, S., Ikenaga, T., Kawakami, K., Ono, F., Hatta, K. (2012) A Gal4 gene trap line useful for studying the morphogenesis of craniofacial perichondrium, vascular endothelium-associated cells, floor plate, and dorsal midline radial glia during zebrafish development. Development Growth and Differentiation 54: 202-215.
Mongeon, R., Walogorsky, M., Urban, J., Mandel, G., Ono, F., Brehm, P. (2011) An acetylcholine receptor lacking both γ and ε subunits mediates transmission in zebrafish slow muscle synapses. Journal of General Physiology 138: 353-366.
Ikenaga, T., Urban, J., Gebhart, N.,
Hatta, K., Kawakami, K., Ono, F. (2011) Formation of the spinal network in
zebrafish determined by domain-specific Pax genes. Journal of Comparative Neurology 519: 1562-1579.
Diep, C., Ma, D., Arora, N., Wingert, R., Bollig, F., Djordjevic, G., Lichman, B., Zhu, H., Ikenaga,
T., Ono, F., Englert, C., Hukriede, N., Handin, R., Davidson, A. (2011) Identification of adult
nephron progenitor cells capable of nephron formation and regeneration in
zebrafish, Nature 470: 95-100.
Won, Y., Ono, F.,
Ikeda, S. (2011) Identification and modulation of voltage-gated Ca2+
current in zebrafish Rohon-Beard neurons, Journal of Neurophysiology 105:
442-453.
Epley, K., Urban, J., Ikenaga, T., and Ono,
F. (2008) A modified acetylcholine receptor d-subunit
enables a null mutant to survive beyond sexual maturation. Journal of
Neuroscience 28: 13223-13231.
Ono, F. (2008) An emerging picture of synapse formation: A
balance of two opposing pathways. Science Signaling 1,
pe3.
Ibanez-Tallon, I., Wen, H., Miwa, J.M., Xing,
J., Tekinay, AB., Ono, F.,
Brehm, P., and Heintz, N. (2004) Tethering naturally
occurring Peptide toxins for cell-autonomous modulation of ion channels and
receptors in vivo. Neuron 43:305-11.
Luna, V., Wang, M., Ono, F., Gleason,
M., Dallman, J., Mandel, G., and Brehm, P. (2004)
Persistent electrical coupling and locomotory
dysfunction in the zebrafish mutant shocked. Journal
of Neurophysiology 92:2003-2009.
Ono, F., Mandel, G., and Brehm, P. (2004) Acetylcholine
receptors direct rapsyn clusters to the neuromuscular synapse in zebrafish. Journal
of Neuroscience 24:5475-5481.
Lefebvre, J.L., Ono, F., Puglielli, C., Saidner, G., Franzini-Armstrong, C., Brehm, P., and Granato,
M. (2004) Increased neuromuscular activity causes axonal defects and muscle
degeneration. Development 31:2605-2618.
Li, W., Ono, F., and Brehm,
P. (2003) Optical measurements of presynaptic release in mutant zebrafish
lacking postsynaptic receptors. Journal of
Neuroscience 23:10467 - 10474.
Nakajo, K., Katsuyama,
Y., Ono, F., Ohtsuka, Y., and Okamura,
Y. (2003) Primary structure, functional characterization and developmental
expression of the ascidian Kv4-class potassium channel. Neuroscience Research 45:59-70.
Ono, F., Shcherbatko, A., Higashijima, S., Mandel, G., and Brehm, P. (2002) The
zebrafish motility mutant "twitch once" reveals new roles for rapsyn
in synaptic function. Journal of
Neuroscience 22:6491-8.
Okada, T., Katsuyama,
Y., Ono, F., and Okamura, Y. (2002) The development of three
identified motor neurons in ascidian embryo. Developmental Biology 244:278-92.
Ono, F., Higashijima, S., Shcherbatko, A., Fetcho, J.R.,
and Brehm, P. (2001) Paralytic zebrafish lacking
acetylcholine receptors fail to localize rapsyn clusters to the synapse. Journal of Neuroscience 21:5439-5448.
Shchberbatko, A., Ono, F., Mandel, G., and Brehm, P. (1999) Voltage-dependent
sodium channel function is regulated through membrane mechanics. Biophysical Journal 77:1945-1959.
Ono, F., Katsuyama, Y., Nakajo,
K., and Okamura, Y. (1999) Subfamily-specific posttranscriptional mechanism
underlies K+ channel expression in a developing neuronal blastomere. Journal of Neuroscience 19:6874-6886.
Okamura, Y., Ono, F. and Okagaki, R. (1997) Regulation of voltage-gated ion channels
during ascidian embryogenesis. Developmental
Neuroscience 19:23-24.
Okamura, Y., Ono, F. and Okagaki, R. (1996) Regulation of sodium channel gene
expression by cell-specific interactions in the Ascidian embryo. Basic Neuroscience in Invertebrates 21-30.
Okamura, Y., Ono, F., Okagaki, R., Chong, J.A., and Mandel, G. (1994) Neural expression
of a sodium channel gene requires cell-specific interactions. Neuron 13:937-948.
レビューアー
American Journal of Physiology, Alcohol,
BBA, Brain Research, Canadian Journal of Zoology, Channels, Developmental
Biology, Developmental Neurobiology, Disease Models & Mechanisms, FASEB
Journal, Journal of Comparative Physiology B, Journal of Drug and Alcohol Research, Journal of Medical Genetics, Journal of Neuroscience,
Journal of Physiology, Molecular Endocrinology, Nature Communications,
Neuropharmacology, Neuroscience Letter, Neuroscience Research, PNAS,
Reproductive Toxicology, Scientific Reports (journals)
The Netherlands Organization for Health
Research and Development, Natural Sciences and Engineering Research Council of
Canada, National Science Foundation, Japan Society for the Promotion of
Science, National Institutes of Health (grants)
講演
Neuroscience Research Institute, Tsukuba,
Japan, June 2002
University of Connecticut Health Center,
Farmington, CT, December 2002
University of Florida, St Augustine &
Gainesville, FL, March 2003
FASEB summer conference "Ion Channel
Regulation", Tucson, AZ, June 2003
Neuroscience Research Institute, Tsukuba,
Japan, June 2004
National Institute of Physiological
Sciences, Okazaki, Japan, June2004
NIH/NIAAA, Rockville, MD, September 2005
Kobe University, Kobe, Japan, July 2006
NICHD, Bethesda, MD, Dec 2009
NIEHS, Research Triangle Park, NC, June
2010
Association of Japanese life scientists (NIH金曜会), Bethesda, MD, June 2010
University of Massachusetts, Amherst, MA,
February 2012
Pennsylvania State University, Hershey,
PA, April 2012
NeuroTalk-2012, Beijing, China, May 2012
10th International Conference on Zebrafish
Development and Genetics, Madison, WI, June 2012
2012 Ion Channel Retreat, Vancouver, Canada, June 2012
University of Rochester, Rochester, NY, Nov 2012
University of Miami, Miami, FL, April 2013
IDARS International Meeting, Mexico City, Mexico, April 2013
University of Tokyo, Tokyo, Japan July 2013
University of Niigata, Niigata, Japan, August 2013
University of Kagoshima, Kagoshima, Japan, August 2013
NIH Research Festival Symposium, Bethesda, MD, November 2013
Osaka Medical College, Osaka, Japan, April
2014
University of Osaka, Osaka, Japan, April 2014
ZFIG meeting, Bethesda, MD April 2014
National Eye Institute, Rockville, MD June
2014
Association of Japanese life scientists (Kinyokai), Bethesda, MD March 2015
Fish biology meeting, Osaka April 2015
BMFT-NIH in
Japan, Nagoya, Japan July 2015
VSP meeting, Kanagawa, Japan November 2015
Ion channel modulation symposium,
Cambridge, UK June 2016
10th European Zebrafish
meeting, Budapest, Hungary July 2017
Jichi Medical College, Japan May 2018
Kyoto Prefectural Medical University,
Japan, May 2018
Sophion Bioscience seminar, Osaka, October 2019
社会活動
Demonstration for
high school students in STUDENT SCIENCE TRAINING PROGRAM at University of
Florida 06/2004
Served
as a judge in Science Fair (8th grade) at Gamble Rogers Middle
School 11/2004
Demonstration
at “Career Fair” at Otis Mason Elementary School 05/2006
Demonstration
for high school students in STUDENT SCIENCE TRAINING PROGRAM at University of
Florida. 06/2006
Demonstration
of zebrafish for “Take Your Child to Work Day” at NIH 04/2008
Demonstration
of zebrafish for “Take Your Child to Work Day” at NIH 04/2009
Demonstration for Jeter’s Leaders at NIH (article in NIH Record) 07/2009
Demonstration
of zebrafish for “Take Your Child to Work Day” at NIH 04/2012
Demonstration for Jeter’s Leaders at NIH 07/2012
Demonstration
of zebrafish for “Take Your Child to Work Day” at NIH 04/2013
Demonstration
of zebrafish for “Take Your Child to Work Day” at NIH 04/2014
高校生に模擬講義 （夢ナビ東京会場、夢ナビ大阪会場） 2016-2019
高槻高校生に実験指導、講義、ポスター審査 2014-2020
IMSPQ(国際生理学クイズ大会)審判 マラヤ大学、インドネシア大学 2017-2019
リンク
Research Map
チュニジアのテレビ放送(NIHのラボが紹介されています)
https://www.youtube.com/watch?v=eLXop76IPas
夢ナビ模擬講義